Coding

Part:BBa_K1067002:Design

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-19)

Cytochrome A and X from Nitrosomonas europaea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal BglII site found at 448
    Illegal BamHI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal NgoMIV site found at 1140
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes taken from Nitrosomonas europaea ATCC 19718 by colony PCR.


References